Rr381^em5Kap
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr381^em5Kap
• Name: regulatory region 381; endonuclease-mediated mutation 5, Dietmar J Kappes
• MGI ID: MGI:7439139
• Synonyms: Sil^Flip250, ThPOK^SilFlip250
• Gene: Rr381 Location: Chr3:89303454-89303824 bp Genetic Position: Chr3, Syntenic
• Alliance: Rr381^em5Kap page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: The Zbtb7b silencer was targeted with an ssODN template, coding for the first 250 bp of the silencer sequence in reverse orientation, using TALEN technology (ZNF target sequences (capitalized) ACCGCTACCCTAACCcataaCTGGAAGGGGTTTAG). The resulting allele has the 5' (distal) end of the silencer flipped, while the 3' (proximal) end, containing a highly conserved noncoding element (HCNE), remains in the normal orientation. (J:333471)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr381 Mutation: 0 strains or lines available

References

• Original: J:333471 Mookerjee-Basu J, et al., Functional Conservation of a Developmental Switch in Mammals since the Jurassic Age. Mol Biol Evol. 2019 Jan 1;36(1):39-53
• All: 1 reference(s)