Cartpt^{tm1.2(cre/ERT2)Hhz}
Targeted Allele Detail

Summary

• Symbol: Cartpt^{tm1.2(cre/ERT2)Hhz}
• Name: CART prepropeptide; targeted mutation 1.2, Herbert Herzog
• MGI ID: MGI:7439824
• Synonyms: CART^-, CART^cre
• Gene: Cartpt Location: Chr13:100034991-100037191 bp, - strand Genetic Position: Chr13, 52.9 cM, cytoband D1
• Alliance: Cartpt^{tm1.2(cre/ERT2)Hhz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333645
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Cartpt^{tm1.2(cre/ERT2)Hhz} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cartpt (MGI:1351330)

• Mutation details: The targeting vector was designed to replace the CART coding exons with the mouse Cartpt cDNA fused to the original CART gene at the initiation codon, which is followed by the insertion of a flippase (Flp) recombinase-recombination target (FRT)-flanked neomycin selection cassette. A cassette carrying the tamoxifen-inducible Cre-recombinase gene followed by an internal ribosome entry site (IRES) that drives the expression of the mCherry red fluorescence reporter gene was inserted. Chimeric offspring were bred with a germline Flp-recombinase mouse line to remove the neomycin cassette. LoxP sites were placed immediately upstream of the initiation codon of the CART and Cre genes respectively, which allows the replacement of the CART gene by Cre. Homozygous mice were then crossed with mice that expressed Cre specifically in the oocytes, resulting in a rearrangement that combines the inducible Cre gene with the endogenous CART promoter. (J:333645)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cartpt (mouse) Summary of all recombinase alleles driven by Cartpt. Comparative matrix view of recombinase activities Alliance mouse Cartpt gene page

Expression

In Mice Carrying this Mutation:

17 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cartpt Mutation: 15 strains or lines available

References

• Original: J:333645 Lau J, et al., Temperature dependence of the control of energy homeostasis requires CART signaling. Neuropeptides. 2016 Oct;59:97-109
• All: 2 reference(s)