Cbln4^{tm1.1(cre/GFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Cbln4^{tm1.1(cre/GFP)Rpa}
• Name: cerebellin 4 precursor protein; targeted mutation 1.1, Richard D Palmiter
• MGI ID: MGI:7439917
• Gene: Cbln4 Location: Chr2:171878256-171885386 bp, - strand Genetic Position: Chr2, 94.74 cM
• Alliance: Cbln4^{tm1.1(cre/GFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331457
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Cbln4^{tm1.1(cre/GFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cbln4 (MGI:2154433)

• Mutation details: A cassette encoding IRES-mnCre:GFP (an internal ribosome entry sequence (IRES), a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 3' of the termination codon in the last coding exon of the Cb;m4 gene via homologous recombination. The neomycin cassette was excised through crosses with animals broadly expressing Flp recombinase. (J:331457)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cbln4 (mouse) Summary of all recombinase alleles driven by Cbln4. Comparative matrix view of recombinase activities Alliance mouse Cbln4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbln4 Mutation: 19 strains or lines available

References

• Original: J:331457 Pauli JL, et al., Molecular and anatomical characterization of parabrachial neurons and their axonal projections. Elife. 2022 Nov 1;11
• All: 1 reference(s)