Rr388^em1Jdai
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr388^em1Jdai
• Name: regulatory region 388; endonuclease-mediated mutation 1, Jianwu Dai
• MGI ID: MGI:7440074
• Synonyms: E3 KO, Eh2^-
• Gene: Rr388 Location: Chr6:64774536-64777054 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr388^em1Jdai page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intergenic deletion
• Mutation details: Atoh1 enhancer Eh2 or E3 was targeted with sgRNAs (targeting TATGAACTAGAATATTGTATTGG and AACGTATTCAAAGTCAAAGCGGG) using CRISPR/Cas9 technology, resulting in a 2519 bp deletion (chr6:64774536-64777054 (GRCm39)) including the enhancer. (J:332270, J:333396)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr388 Mutation: 0 strains or lines available

References

• Original: J:332270 Shu M, et al., Single-cell chromatin accessibility identifies enhancer networks driving gene expression during spinal cord development in mouse. Dev Cell. 2022 Dec 19;57(24):2761-2775.e6
• All: 2 reference(s)