Fgf8^{em1(flpo)Thore}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fgf8^{em1(flpo)Thore}
• Name: fibroblast growth factor 8; endonuclease-mediated mutation 1, Wallace Thoreson
• MGI ID: MGI:7441912
• Synonyms: Fgf8-P2A-FlpO, Fgf8^P2A-FlpO
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^{em1(flpo)Thore} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Fgf8^{em1(flpo)Thore} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fgf8 (MGI:99604)

• Mutation details: Using CRISPR/Cas9-directed genome editing, P2A-FlpO was inserted into the last coding exon of the Fgf8 gene. (J:263636)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fgf8 (mouse) Summary of all recombinase alleles driven by Fgf8. Comparative matrix view of recombinase activities Alliance mouse Fgf8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 21 strains or lines available

References

• Original: J:263636 Quadros RM, et al., Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol. 2017 May 17;18(1):92
• All: 1 reference(s)