Cda^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cda^{tm1d(EUCOMM)Wtsi}
• Name: cytidine deaminase; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7442520
• Synonyms: Cda^-, Cda KO
• Gene: Cda Location: Chr4:138065735-138095303 bp, - strand Genetic Position: Chr4, 70.17 cM, cytoband D3
• Alliance: Cda^{tm1d(EUCOMM)Wtsi} page
• IMPC: Cda gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332259
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 138078265 of Chromosome 4 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 138079066. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:332259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cda Mutation: 8 strains or lines available

References

• Original: J:332259 Onclercq-Delic R, et al., Cytidine deaminase deficiency in mice enhances genetic instability but limits the number of chemically induced colon tumors. Cancer Lett. 2022 Dec 8;555:216030
• All: 1 reference(s)