Cmtm4^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cmtm4^{tm1d(EUCOMM)Wtsi}
• Name: CKLF-like MARVEL transmembrane domain containing 4; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7442597
• Synonyms: Cmtm4^-
• Gene: Cmtm4 Location: Chr8:105074823-105122439 bp, - strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cmtm4^{tm1d(EUCOMM)Wtsi} page
• IMPC: Cmtm4 gene page

Mutation origin

• Mutant Cell Line: EPD0495_1_A04
• Germline Transmission: Earliest citation of germline transmission: J:333836
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 105082606 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 105084981. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333836)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cmtm4 Mutation: 12 strains or lines available

References

• Original: J:333836 Knizkova D, et al., CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology. Nat Immunol. 2022 Nov;23(11):1644-1652
• All: 1 reference(s)