Xcr1^{tm2.1(icre)Ciphe}
Targeted Allele Detail

Summary

• Symbol: Xcr1^{tm2.1(icre)Ciphe}
• Name: chemokine (C motif) receptor 1; targeted mutation 2.1, Centre d'ImmunoPhenomique
• MGI ID: MGI:7442771
• Synonyms: Xcr1^Cre-mTFP1, Xcr1-IRES-iCre-GSG-2A-mTFP1, Xcr1^tm2Ciphe
• Gene: Xcr1 Location: Chr9:123681380-123691094 bp, - strand Genetic Position: Chr9, 74.71 cM, cytoband F4
• Alliance: Xcr1^{tm2.1(icre)Ciphe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327136
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Xcr1^{tm2.1(icre)Ciphe} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Xcr1 (MGI:1346338)

• Mutation details: A targeting construct was designed to introduce an IRES-iCre-GSG-2A-mTFP1 cassette in the 3 untranslated region of the Xcr1gene, 29base pair (bp) downstream of the stop codon. mTFP1 is the sequence coding for a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein. After germline transmission, excision of the frt-neor-frt cassette was achieved through crossing with mice expressing an FLP recombinase (J:327136)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Xcr1 (mouse) Summary of all recombinase alleles driven by Xcr1. Comparative matrix view of recombinase activities Alliance mouse Xcr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xcr1 Mutation: 33 strains or lines available

References

• Original: J:327136 Wohn C, et al., Absence of MHC class II on cDC1 dendritic cells triggers fatal autoimmunity to a cross-presented self-antigen. Sci Immunol. 2020 Mar 13;5(45):eaba1896
• All: 11 reference(s)