Atoh1^{tm2.1(cre/ERT2,-CD2)Dwi}
Targeted Allele Detail

Summary

• Symbol: Atoh1^{tm2.1(cre/ERT2,-CD2)Dwi}
• Name: atonal bHLH transcription factor 1; targeted mutation 2.1, Douglas J Winton
• MGI ID: MGI:7444368
• Synonyms: Atoh1^{(9S/T-A)CreERT2}
• Gene: Atoh1 Location: Chr6:64706109-64708229 bp, + strand Genetic Position: Chr6, 30.03 cM
• Alliance: Atoh1^{tm2.1(cre/ERT2,-CD2)Dwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271399
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Dominant negative, Epitope tag, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intergenic deletion
• Atoh1^{tm2.1(cre/ERT2,-CD2)Dwi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Atoh1 (MGI:104654)

• Mutation details: The targeting construct was designed to introduce a loxP site into 5'UTR of Atoh1, followed by the hyperactive phosphomutant (9 amino acids S/T to A changes) Atoh1 sequence Atoh1(9S/T-A) merged to the P2A-CreERT2-hCD2 then to a neomycin cassette inserted such that the 3'UTR was not disrupted. Following successful germline transmission, the mice heterozygous for the targeting construct were crossed onto PGK-Cre line in order to remove both the neo-selection cassette and the endogenous Atoh1 locus at the same time. (J:271399)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Atoh1 (mouse) Summary of all recombinase alleles driven by Atoh1. Alliance mouse Atoh1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atoh1 Mutation: 37 strains or lines available

References

• Original: J:271399 Tomic G, et al., Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration. Cell Stem Cell. 2018 Sep 6;23(3):436-443.e7
• All: 1 reference(s)