4930590J08Rik^em1Qsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: 4930590J08Rik^em1Qsh
• Name: RIKEN cDNA 4930590J08 gene; endonuclease-mediated mutation 1, Qinghua Shi
• MGI ID: MGI:7444374
• Synonyms: delta26
• Gene: 4930590J08Rik Location: Chr6:91879790-91927706 bp, + strand Genetic Position: Chr6, 40.94 cM
• Alliance: 4930590J08Rik^em1Qsh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 26-bp deletion in exon 9. The mutation resulted in a frameshift and a premature translation termination codon, which is predicted to result in the production of a truncated protein. Direct sequencing of testis cDNA confirmed the 26-bp deletion in homozygous mutant mice. (J:333195)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any 4930590J08Rik Mutation: 34 strains or lines available

References

• Original: J:333195 Chen R, et al., Impaired fertility in 4930590J08Rik mutant male mice is associated with defective sperm energy metabolism. FASEB J. 2022 Dec;36(12):e22634
• All: 1 reference(s)