Esm1^{em1(icre/ERT2)Ben}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Esm1^{em1(icre/ERT2)Ben}
• Name: endothelial cell-specific molecule 1; endonuclease-mediated mutation 1, Rui Benedito
• MGI ID: MGI:7444426
• Synonyms: Esm1-HA-H2B-Cerulean-2A-CreERT2, Gt(Esm1)^{tm(HA-H2B-Cerulean-2A-CreERT2)}
• Gene: Esm1 Location: Chr13:113346193-113354632 bp, + strand Genetic Position: Chr13, 63.93 cM
• Alliance: Esm1^{em1(icre/ERT2)Ben} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275593
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Esm1^{em1(icre/ERT2)Ben} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Esm1 (MGI:1918940)

• Mutation details: Using CRISPR/Cas9-assisted homologous-dependent recombination in mouse ES cells, the HA-H2B-Cerulean-2A-iCreERT2 cassette was inserted in-frame with the endogenous Esm1 gene ATG initiation codon. (J:275593)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Esm1 (mouse) Summary of all recombinase alleles driven by Esm1. Comparative matrix view of recombinase activities Alliance mouse Esm1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Esm1 Mutation: 16 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:275593 Pontes-Quero S, et al., High mitogenic stimulation arrests angiogenesis. Nat Commun. 2019 May 1;10(1):2016
• All: 1 reference(s)