Ythdf2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ythdf2^{tm1c(KOMP)Wtsi}
• Name: YTH N6-methyladenosine RNA binding protein 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7444511
• Gene: Ythdf2 Location: Chr4:131912227-131939567 bp, - strand Genetic Position: Chr4, 64.92 cM
• Alliance: Ythdf2^{tm1c(KOMP)Wtsi} page
• IMPC: Ythdf2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333887
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 131931215 of Chromosome 4 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 131933516. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:324152, J:333887)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ythdf2 Mutation: 32 strains or lines available

References

• Original: J:333887 Wu Y, et al., N(6)-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition. Nat Cell Biol. 2022 Jun;24(6):917-927
• All: 2 reference(s)