Hacd2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Hacd2^{tm1c(EUCOMM)Hmgu}
• Name: 3-hydroxyacyl-CoA dehydratase 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7444803
• Synonyms: Hacd2^cKO
• Gene: Hacd2 Location: Chr16:34842798-34929547 bp, + strand Genetic Position: Chr16, 24.54 cM
• Alliance: Hacd2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Hacd2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0663_6_A10, HEPD0663_6_G09
• Germline Transmission: Earliest citation of germline transmission: J:333944
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34845737 of Chromosome 16 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 34846568. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333944)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hacd2 Mutation: 18 strains or lines available

References

• Original: J:333944 Khadhraoui N, et al., Hacd2 deficiency in mice leads to an early and lethal mitochondrial disease. Mol Metab. 2023 Mar;69:101677
• All: 1 reference(s)