Hacd2^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Hacd2^{tm1d(EUCOMM)Hmgu}
• Name: 3-hydroxyacyl-CoA dehydratase 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7444806
• Synonyms: Hacd2^completeKO
• Gene: Hacd2 Location: Chr16:34842798-34929547 bp, + strand Genetic Position: Chr16, 24.54 cM
• Alliance: Hacd2^{tm1d(EUCOMM)Hmgu} page
• IMPC: Hacd2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0663_6_A10, HEPD0663_6_G09
• Germline Transmission: Earliest citation of germline transmission: J:333944
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34845737 of Chromosome 16 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 34846568. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333944)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hacd2 Mutation: 18 strains or lines available

References

• Original: J:333944 Khadhraoui N, et al., Hacd2 deficiency in mice leads to an early and lethal mitochondrial disease. Mol Metab. 2023 Mar;69:101677
• All: 1 reference(s)