Agrp^{tm4.1(cre/EGFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Agrp^{tm4.1(cre/EGFP)Rpa}
• Name: agouti related neuropeptide; targeted mutation 4.1, Richard D Palmiter
• MGI ID: MGI:7448510
• Synonyms: AgrpCre:GFP(version 2)
• Gene: Agrp Location: Chr8:106293327-106306477 bp, - strand Genetic Position: Chr8, 53.04 cM, cytoband D1-D2
• Alliance: Agrp^{tm4.1(cre/EGFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:295967
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Recombinase, Reporter)
• Mutation: Insertion
• Agrp^{tm4.1(cre/EGFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Agrp (MGI:892013)

• Mutation details: The targeting construct introduced a cassette containing the following features, without the nuclear localization signal, using a nonoptimal initiation codon, a cre recombinase gene fused with EGFP (cre/EGFP) with N-term sequence removed, and a myc tag with an additional 3 untranslated region from the Myc gene that promotes a short messenger RNA half-life. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette introduced downstream of the fusion gene. It is a revised "version 2" of the allele Agrp^{tm1.1(cre/EGFP)Stei} designed to have an attenuated expression of Cre:GFP. (J:295967)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Agrp (mouse) Summary of all recombinase alleles driven by Agrp. Comparative matrix view of recombinase activities Alliance mouse Agrp gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agrp Mutation: 17 strains or lines available

References

• Original: J:295967 Ewbank SN, et al., Chronic Gq signaling in AgRP neurons does not cause obesity. Proc Natl Acad Sci U S A. 2020 Aug 25;117(34):20874-20880
• All: 1 reference(s)