Tg(Nes-cre/ERT2,-EGFP,-HBEGF)1Lfp
Transgene Detail

Summary

• Symbol: Tg(Nes-cre/ERT2,-EGFP,-HBEGF)1Lfp
• Name: transgene insertion 1, Luis F Parada
• MGI ID: MGI:7449234
• Synonyms: CGD
• Transgene: Tg(Nes-cre/ERT2,-EGFP,-HBEGF)1Lfp Location: unknown
• Alliance: Tg(Nes-cre/ERT2,-EGFP,-HBEGF)1Lfp page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inducible, Inserted expressed sequence, Recombinase, Reporter)
• Mutation: Insertion
• Tg(Nes-cre/ERT2,-EGFP,-HBEGF)1Lfp expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Tg(Nes-cre/ERT2,-EGFP,-HBEGF)1Lfp expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nes (MGI:101784)

• Mutation details: The 5.6 kb of the rat 5' regulatory element followed by CreERT2, histone 2B tagged enhanced GFP, and human diphtheria toxin receptor (CGD), each element separated by p2A sequences, and 668bp of the inverted second intron were used to express three proteins from one transcript in neural stem cells. Twenty-nine transgenic founder lines were identified. A single founder that displayed the specific expression pattern without detectable ectopic expression was established. (J:299260)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (mouse) Summary of all recombinase alleles driven by Nes. Comparative matrix view of recombinase activities Alliance mouse Nes gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:299260 Xie XP, et al., High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging. Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31448-31458
• All: 2 reference(s)