Kcnt1^em1Nju
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kcnt1^em1Nju
• Name: potassium channel, subfamily T, member 1; endonuclease-mediated mutation 1, Model Animal Research Center of Nanjing University
• MGI ID: MGI:7449379
• Synonyms: Slack-Y777H
• Gene: Kcnt1 Location: Chr2:25753807-25808285 bp, + strand Genetic Position: Chr2, 18.27 cM
• Alliance: Kcnt1^em1Nju page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: Tyrosine codon 777 (TAT) in exon 21 was changed to histidine (CAT) (p.Y777H) using an sgRNA and an ssODN template with CRISPR/Cas9 techology. This is a gain-of-function mutation that increases the sodium channel's sensitivity to sodium. (J:333464)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnt1 Mutation: 67 strains or lines available

References

• Original: J:333464 Zhang Q, et al., The Slack Channel Regulates Anxiety-Like Behaviors via Basolateral Amygdala Glutamatergic Projections to Ventral Hippocampus. J Neurosci. 2022 Apr 6;42(14):3049-3064
• All: 1 reference(s)