P2ry12^{em1(cre/ERT2)Peng}
Endonuclease-mediated Allele Detail

Summary

• Symbol: P2ry12^{em1(cre/ERT2)Peng}
• Name: purinergic receptor P2Y, G-protein coupled 12; endonuclease-mediated mutation 1, Bo Peng
• MGI ID: MGI:7450934
• Synonyms: P2Y12-CreER-GFP
• Gene: P2ry12 Location: Chr3:59123693-59170292 bp, - strand Genetic Position: Chr3, 29.01 cM
• Alliance: P2ry12^{em1(cre/ERT2)Peng} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• P2ry12^{em1(cre/ERT2)Peng} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	P2ry12 (MGI:1918089)

• Mutation details: Using CRISPR/Cas9-based extreme genome editing, a p2A-creERT2-p2A-EGFP cassette was inserted between the last exon of the P2ry12 gene and its 3 UTR. (J:330602)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	P2ry12 (mouse) Summary of all recombinase alleles driven by P2ry12. Comparative matrix view of recombinase activities Alliance mouse P2ry12 gene page

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2ry12 Mutation: 41 strains or lines available

References

• Original: J:330602 Zhou T, et al., Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice. Nat Commun. 2022 Oct 24;13(1):6233
• All: 1 reference(s)