Map3k7^tm1.1Arte
Targeted Allele Detail

Summary

• Symbol: Map3k7^tm1.1Arte
• Name: mitogen-activated protein kinase kinase kinase 7; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:7450973
• Synonyms: Map3k7^flox
• Gene: Map3k7 Location: Chr4:31964097-32023467 bp, + strand Genetic Position: Chr4, 13.91 cM
• Alliance: Map3k7^tm1.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332172
• Parent Cell Line: ART B6 3.6 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: The following sequence was inserted into intron 5: a loxP site, an FRT site flanked neomycin resistance gene cassette, the 3' end of Map3k7 intron 5, Map3k7 exons 6-17 cDNA, human GH poly(A) signal sequences, and an F3 site flanked puromycin resistance gene cassette. Exon 6 was replaced with a version carrying a mutation changing aspartic acid codon 175 (GAT) to alanine (GCT) (p.D175A). The neo and puro cassettes were removed through subsequent Flp-mediated recombination. This allele will express the wild-type peptide from the inserted mini-gene unless conditional Cre-mediated deletion removes the mini-gene, in which case the peptide with the D175A mutation will be expressed. The mutation renders the peptide kinase-dead. (J:332172)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k7 Mutation: 54 strains or lines available

References

• Original: J:332172 Rodrigues M, et al., TAK1 protein kinase activity is required for TLR signalling and cytokine production in myeloid cells. Biochem J. 2022 Sep 16;479(17):1891-1907
• All: 1 reference(s)