Tssk3^em2Amsa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tssk3^em2Amsa
• Name: testis-specific serine kinase 3; endonuclease-mediated mutation 2, Ana M Salicioni
• MGI ID: MGI:7451037
• Synonyms: Tssk3^- Line 2
• Gene: Tssk3 Location: Chr4:129382807-129384815 bp, - strand Genetic Position: Chr4, 63.26 cM, cytoband D2.3
• Alliance: Tssk3^em2Amsa page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 6 bp deletion (CCAATG) and a 19 bp insertion (AATTGGTATCAGCTCTACT) in exon 1 resulting in a frameshift mutation and an early stop codon. Absence of the targeted region was confirmed by PCR and Sanger sequencing. (J:334491)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tssk3 Mutation: 10 strains or lines available

References

• Original: J:334491 Nayyab S, et al., TSSK3, a novel target for male contraception, is required for spermiogenesis. Mol Reprod Dev. 2021 Nov;88(11):718-730
• All: 1 reference(s)