Lgr5^{em1(cre/ERT2)Kba}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lgr5^{em1(cre/ERT2)Kba}
• Name: leucine rich repeat containing G protein coupled receptor 5; endonuclease-mediated mutation 1, Konrad Basler
• MGI ID: MGI:7460264
• Synonyms: Lgr5-2A-CreERT2-2A-mOrange2, Lgr5^ZH
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{em1(cre/ERT2)Kba} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Lgr5^{em1(cre/ERT2)Kba} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lgr5 (MGI:1341817)

• Mutation details: CRISPR-targeting introduced a sequence containing an Lgr5-2A-CreERT2-2A-mOrange2 at the 3 end of the Lgr5 gene. (J:334516)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities Alliance mouse Lgr5 gene page

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 58 strains or lines available

References

• Original: J:334516 Reichmuth J, et al., Manipulating the murine Lgr5 locus using a rapid, efficient and flexible CRISPR/Cas9 pipeline. bioRxiv. 2020
• All: 2 reference(s)