Eif4a2^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Eif4a2^em1(IMPC)J
• Name: eukaryotic translation initiation factor 4A2; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:7461814
• Gene: Eif4a2 Location: Chr16:22926194-22932886 bp, + strand Genetic Position: Chr16, 13.93 cM
• Alliance: Eif4a2^em1(IMPC)J page
• IMPC: Eif4a2 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GCGTTTTCAGCAGAGCACAG and ATCCACCCTGAGAAACCTAT, which resulted in a 995 bp deletion beginning at Chromosome 16 position 23,108,486 bp and ending after 23,109,480 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001306288, ENSMUSE00001235294 and ENSMUSE00000560683 (exons 2, 3 and 4) and 676 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 10 and early truncation 3 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Eif4a2 Mutation: 29 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 2 reference(s)