Npff^{tm1.1(icre)Xen}
Targeted Allele Detail

Summary

• Symbol: Npff^{tm1.1(icre)Xen}
• Name: neuropeptide FF-amide peptide precursor; targeted mutation 1.1, Taconic Biosciences
• MGI ID: MGI:7464745
• Synonyms: NPFF^Cre
• Gene: Npff Location: Chr15:102432274-102433056 bp, - strand Genetic Position: Chr15, 57.65 cM, cytoband F3
• Alliance: Npff^{tm1.1(icre)Xen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334879
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Npff^{tm1.1(icre)Xen} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Npff (MGI:1891708)

• Mutation details: Using a conventional ES cell targeting strategy and homologous recombination, the T2A peptide and the open reading frame of improved Cre recombinase (iCre) were inserted between the last amino acid and the translation termination codon in exon 3 of the Npff gene. A positive selection marker (Puromycin resistance) flanked by FRT sites was removed by crossing with germline Flpe mice. (J:334879)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Npff (mouse) Summary of all recombinase alleles driven by Npff. Comparative matrix view of recombinase activities Alliance mouse Npff gene page

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Npff Mutation: 6 strains or lines available

References

• Original: J:334879 Quillet R, et al., Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord. Sci Rep. 2023 Apr 11;13(1):5891
• All: 1 reference(s)