Poglut2^{tm2d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Poglut2^{tm2d(EUCOMM)Hmgu}
• Name: protein O-glucosyltransferase 2; targeted mutation 2d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7465080
• Gene: Poglut2 Location: Chr1:44145706-44157968 bp, - strand Genetic Position: Chr1, 23.54 cM, cytoband C1
• Alliance: Poglut2^{tm2d(EUCOMM)Hmgu} page
• IMPC: Poglut2 gene page

Mutation origin

• Mutant Cell Line: HEPD0770_7_B12
• Germline Transmission: Earliest citation of germline transmission: J:157065
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 44153334 of Chromosome 1 upstream of the critical exons 3-4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3-4 at position 44154510. The critical exons 3-4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse with exons 3-4 deleted. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:350484)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Poglut2 Mutation: 27 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)