Poglut3^tm1.3Rsh
Targeted Allele Detail

Summary

• Symbol: Poglut3^tm1.3Rsh
• Name: protein O-glucosyltransferase 3; targeted mutation 1.3, Robert Haltiwanger
• MGI ID: MGI:7465087
• Gene: Poglut3 Location: Chr9:53295325-53313167 bp, + strand Genetic Position: Chr9, 29.12 cM
• Alliance: Poglut3^tm1.3Rsh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:350484
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct PG00252_A_1_B04 inserted upstream of the critical exons 2-3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2-3 at position 44154510. The critical exons 2-3 are thus flanked by loxP sites. Flp-mediated recombination deleted the lacZ and neomcyin resistance gene, generating a "conditional ready" exon 2-3 floxed allele. Subsequent cre-mediated recombination deleted exons 2-3 and generated a knock-out allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:350484)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Poglut3 Mutation: 19 strains or lines available

References

• Original: J:350484 Neupane S, et al., Poglut2/3 double knockout in mice results in neonatal lethality with reduced levels of fibrillin in lung tissues. J Biol Chem. 2024 Jun 4;300(7):107445
• All: 1 reference(s)