Aldh1a1^em2(cre)Ylu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Aldh1a1^em2(cre)Ylu
• Name: aldehyde dehydrogenase family 1, subfamily A1; endonuclease-mediated mutation 2, Youming Lu
• MGI ID: MGI:7466201
• Synonyms: Aldh1a1^-/--CRE
• Gene: Aldh1a1 Location: Chr19:20470079-20620829 bp, + strand Genetic Position: Chr19, 13.91 cM
• Alliance: Aldh1a1^em2(cre)Ylu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Aldh1a1^em2(cre)Ylu expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aldh1a1 (MGI:1353450)

• Mutation details: Using CRISPR/Cas9 system, P2A-CRE-pA was inserted downstream of exon 10 of the Aldh1a1 gene. The premature termination of the transcript deleted the exons 1113 corresponding to the 100 amino acids (401-500) of the C-terminal, which is essential for enzyme function and stability of Aldh1a1. It is a null allele. The absence of protein products was established by western blot analysis. (J:316743)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aldh1a1 (mouse) Summary of all recombinase alleles driven by Aldh1a1. Comparative matrix view of recombinase activities Alliance mouse Aldh1a1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aldh1a1 Mutation: 39 strains or lines available

References

• Original: J:316743 Li X, et al., Synaptic dysfunction of Aldh1a1 neurons in the ventral tegmental area causes impulsive behaviors. Mol Neurodegener. 2021 Oct 26;16(1):73
• All: 1 reference(s)