Igs51^{tm1(CAG-flpe,-EGFP)Itoi}
Targeted Allele Detail

Summary

• Symbol: Igs51^{tm1(CAG-flpe,-EGFP)Itoi}
• Name: intergenic site 51; targeted mutation 1, Keiichi Itoi
• MGI ID: MGI:7466667
• Synonyms: Actb-FLPe, FLP deleter
• Gene: Igs51 Location: unknown Genetic Position: Chr5, Syntenic
• Alliance: Igs51^{tm1(CAG-flpe,-EGFP)Itoi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279641
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag, Recombinase, Reporter)
• Mutation: Insertion
• Igs51^{tm1(CAG-flpe,-EGFP)Itoi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The following elements were inserted downstream of the Actb gene in intergenic region Igs51 via homologous recombination: CAG promoter, the flpe recombinase gene, IRES sequences, N-terminal FLAG tagged EGFP reporter gene, SV40 poly(A) signal sequences, a loxP site, a neomycin resistance gene cassette, and a second loxP site. (J:279641)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs51 Mutation: 0 strains or lines available

References

• Original: J:279641 Kono J, et al., Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse. Brain Struct Funct. 2017 May;222(4):1705-1732
• All: 4 reference(s)