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Pold1tm1b(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Pold1tm1b(EUCOMM)Wtsi
Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:7467227
Gene: Pold1  Location: Chr7:44182168-44198239 bp, - strand  Genetic Position: Chr7, 28.83 cM
Alliance: Pold1tm1b(EUCOMM)Wtsi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:327432
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 44189712 of Chromosome 7 into intron 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted into intron 10 at position 44192163. Exons 4-10 are thus flanked by loxP sites. Cre expression generated a reporter knockout mouse, deleting exons 4-10. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:327432)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pold1 Mutation:  63 strains or lines available
References
Original:  J:327432 Chen T, et al., A hypomorphic mutation in Pold1 disrupts the coordination of embryo size expansion and morphogenesis during gastrulation. Biol Open. 2022 Aug 15;11(8):bio059307
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory