Phactr1^tm1.2Gdo
Targeted Allele Detail

Summary

• Symbol: Phactr1^tm1.2Gdo
• Name: phosphatase and actin regulator 1; targeted mutation 1.2, Gillian Douglas
• MGI ID: MGI:7469027
• Gene: Phactr1 Location: Chr13:42834099-43292002 bp, + strand Genetic Position: Chr13, 21.05 cM
• Alliance: Phactr1^tm1.2Gdo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335230
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Targeting vector HTGRS6013_A_D10 from the Knock-out Mouse Project inserted an FRT flanked IRES-lacZ-pA cassette linked to a strong splice acceptor signal, together with a loxP flanked neomycin selection cassette into intron 6 and an additional loxP site into intron 7, thus flanking exon 7 with loxP sites. The lacZ cassette was removed via flp-mediated recombination. Exon 7 was deleted via cre-mediated recombination in the germline. (J:335230)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phactr1 Mutation: 43 strains or lines available

References

• Original: J:335230 Wood A, et al., PHACTR1 modulates vascular compliance but not endothelial function: a translational study. Cardiovasc Res. 2023 Mar 31;119(2):599-610
• All: 1 reference(s)