Pou4f3^{tm1.2(cre)Tcba}
Targeted Allele Detail

Summary

• Symbol: Pou4f3^{tm1.2(cre)Tcba}
• Name: POU domain, class 4, transcription factor 3; targeted mutation 1.2, Tudor Constantin Badea
• MGI ID: MGI:7470766
• Synonyms: Brn3c^Cre
• Gene: Pou4f3 Location: Chr18:42527662-42529158 bp, + strand Genetic Position: Chr18, 22.58 cM, cytoband B3-E1
• Alliance: Pou4f3^{tm1.2(cre)Tcba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:322005
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion, Inversion
• Pou4f3^{tm1.2(cre)Tcba} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pou4f3 (MGI:102523)

• Mutation details: The targeting consists of a FREX cassette (containing the rox12 and roxP target sites for the Dre recombinase in direct orientation, separated by a 68 bp spacer) inserted in the 5' untranslated region (UTR) of the Pou4f3 locus, 65 bp before the initiator codon ATG; three repeats of the SV40 early region transcription terminator inserted in the 3' UTR, 564 bp downstream of the STOP codon; the Cre recombinase cDNA in reverse orientation and a second FREX cassette in reverse orientation; and the Neomycin positive selection cassette (PGKNeo) flanked by FRT (Flp recombinase target) sites. The targeting vector was introduced to 129-derived R1 embryonic stem cells. The neomycin cassette was excised with a germline FLP recombinase. Dre-mediated recombination generated inversion-excision at this locus results in the expression of Cre from the Pou4f3 promoter. (J:322005)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pou4f3 (mouse) Summary of all recombinase alleles driven by Pou4f3. Comparative matrix view of recombinase activities Alliance mouse Pou4f3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pou4f3 Mutation: 25 strains or lines available

References

• Original: J:322005 Parmhans N, et al., Identification of retinal ganglion cell types and brain nuclei expressing the transcription factor Brn3c/Pou4f3 using a Cre recombinase knock-in allele. J Comp Neurol. 2021 Jun;529(8):1926-1953
• All: 1 reference(s)