Zfp750^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zfp750^{tm1b(EUCOMM)Wtsi}
• Name: zinc finger protein 750; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7470773
• Synonyms: Zfp750^-
• Gene: Zfp750 Location: Chr11:121401804-121410159 bp, - strand Genetic Position: Chr11, 85.49 cM
• Alliance: Zfp750^{tm1b(EUCOMM)Wtsi} page
• IMPC: Zfp750 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335426
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 121402540 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 121405502. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:335426)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp750 Mutation: 28 strains or lines available

References

• Original: J:335426 Butera A, et al., ZFP750 affects the cutaneous barrier through regulating lipid metabolism. Sci Adv. 2023 Apr 28;9(17):eadg5423
• All: 1 reference(s)