Trim28^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Trim28^tm1.1Ics
• Name: tripartite motif-containing 28; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:7471137
• Synonyms: Trim28^C651F, Trim28^PHD
• Gene: Trim28 Location: Chr7:12758079-12764959 bp, + strand Genetic Position: Chr7, 7.73 cM, cytoband A2
• Alliance: Trim28^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327192
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Cysteine codon 651 (TGC) in exon 13 was changed to phenylalanine (TTT) (p.C651F) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 3. The neo cassette was removed through subsequent Cre-mediated recombination. The mutation is located in the PHD domain of the encoded peptide and abrogates its SUMO-E3-ligase activity. (J:327192)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trim28 Mutation: 36 strains or lines available

References

• Original: J:327192 Rossitto M, et al., TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway. Nat Commun. 2022 Jul 29;13(1):4412
• All: 1 reference(s)