Rpl3^em1Kei
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rpl3^em1Kei
• Name: ribosomal protein L3; endonuclease-mediated mutation 1, Keiichi I Nakayama
• MGI ID: MGI:7471434
• Gene: Rpl3 Location: Chr15:79961993-79967553 bp, - strand Genetic Position: Chr15, 37.85 cM
• Alliance: Rpl3^em1Kei page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology was used to delete exon 2. Reverse transcription and quantitative PCR analysis of heart and skeletal muscle confirmed that mRNA is either undetectable for the deleted region or reduced in abundance for the 3UTR, likely due to nonsense-mediated mRNA decay. Mass spectrometry-based multiple reaction monitoring confirmed absence of protein in the heart. (J:335523)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rpl3 Mutation: 35 strains or lines available

References

• Original: J:335523 Shiraishi C, et al., RPL3L-containing ribosomes determine translation elongation dynamics required for cardiac function. Nat Commun. 2023 Apr 20;14(1):2131
• All: 1 reference(s)