Tg(Camk2a-cre/ERT2/Hif1a)#Heh
Transgene Detail

Summary

• Symbol: Tg(Camk2a-cre/ERT2/Hif1a)#Heh
• Name: transgene insertion, Hannelore Ehrenreich
• MGI ID: MGI:7481236
• Synonyms: CamkIIa-CreERT2-ODD
• Transgene: Tg(Camk2a-cre/ERT2/Hif1a)#Heh Location: unknown
• Alliance: Tg(Camk2a-cre/ERT2/Hif1a)#Heh page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen and hypoxia
• Mutation: Insertion
• Tg(Camk2a-cre/ERT2/Hif1a)#Heh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Camk2a (MGI:88256)

• Mutation details: The transgene containing the murine Camk2a promoter drives expression of the tamoxifen-inducible cre recombinase gene (cre/ERT2) fused with the oxygen-dependent degradation (ODD) domain of Hif1a. This transgene is responsive to hypoxia. Under normoxic conditions, the ODD is hydroxylated by prolylhydroxylases, which tag HIF-1 upon binding of Von Hippel-Lindau (VHL) protein for proteasomal degradation. Hypoxic conditions lead to the inactivation of these enzymes and thus stabilization of HIF-1, allowing it to accumulate in the nucleus and activate the transcription of its target genes. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:322009)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Camk2a (mouse) Summary of all recombinase alleles driven by Camk2a. Comparative matrix view of recombinase activities Alliance mouse Camk2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:322009 Butt UJ, et al., CaMKIIalpha Expressing Neurons to Report Activity-Related Endogenous Hypoxia upon Motor-Cognitive Challenge. Int J Mol Sci. 2021 Mar 20;22(6)
• All: 1 reference(s)