Igs7tm203(tetO-sChroME*/GCaMP7s,CAG-mRuby3,-tTA2)Tasic
Targeted Allele Detail
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Symbol: |
Igs7tm203(tetO-sChroME*/GCaMP7s,CAG-mRuby3,-tTA2)Tasic |
Name: |
intergenic site 7; targeted mutation 203, Bosiljka Tasic |
MGI ID: |
MGI:7482000 |
Synonyms: |
Ai203, Ai203TITL-sChroME-Kv2.1-GC7s-WPRE-ICL-nls-mRuby3-IRES2-tTA2-WPRE |
Gene: |
Igs7 Location: unknown Genetic Position: Chr9, Syntenic
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Alliance: |
Igs7tm203(tetO-sChroME*/GCaMP7s,CAG-mRuby3,-tTA2)Tasic page
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Allele Type: |
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Targeted (Conditional ready, Inducible, Reporter, Transactivator) |
Mutation: |
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Insertion
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Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette and a sChroME*/GCaMP7s-WPRE sequence that is followed by a CAG promoter, Lox2272-STOP-Lox2272 cassette, mRuby3, and IRES-tTA2-WPRE sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high cre and/or Tet inducibility). sChroME*/GCaMP7s is the result of a sChroME* coupled to the fluorescent reporter GCaMP7s (an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with slow decay and response kinetics). sChroME is a soma-targeted (ST) ultrafast and highly potent opsin with 3-5x larger photocurrents than opsins commonly used for multiphoton optogenetics. Of note, the Ai203 allele was designed with an IRES upstream of the tTA2 to reduce/eliminate leakiness in the absence of cre recombinase.
(J:339333)
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Original: |
J:339333 Bounds HA, et al., All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse. Cell Rep. 2023 Aug 4;42(8):112909 |
All: |
1 reference(s) |
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