Igs7tm228(tetO-GCaMP6m,CAG-ChRmine*/oScarlet*,CAG-tTA2)Tasic
Targeted Allele Detail
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Symbol: |
Igs7tm228(tetO-GCaMP6m,CAG-ChRmine*/oScarlet*,CAG-tTA2)Tasic |
Name: |
intergenic site 7; targeted mutation 228, Bosiljka Tasic |
MGI ID: |
MGI:7482006 |
Synonyms: |
Ai228, Ai228TIT2L-GCaMP6m-WPRE-ICL2272-ChRmine-oScarlet-Kv2.1-ER-WPRE-ICLoxN-IRES-tTA2-WPRE |
Gene: |
Igs7 Location: unknown Genetic Position: Chr9, Syntenic
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Alliance: |
Igs7tm228(tetO-GCaMP6m,CAG-ChRmine*/oScarlet*,CAG-tTA2)Tasic page
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Allele Type: |
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Targeted (Conditional ready, Inducible, Reporter, Transactivator) |
Mutation: |
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Insertion
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Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette, GCaMP6m, a CAG promoter, Lox2272-STOP-Lox2272 cassette, ChRmine*/oScarlet* sequence, and then a CAG promoter, LoxN-STOP-LoxN cassette, and IRES-tTA2 sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high Cre and/or Tet inducibility). ). ChRmine is a pump-like cation-conducting channelrhodopsin, derived from Rhodomonas lens, that exhibits extremely high current and light sensitivity, as well as a markedly red-shifted spectrum. This is fused with a membrane trafficking signal (TS) and oScarlet fluorescent protein, which is based on the sequence of mScarlet with an additional E95D mutation to increase neuronal cell trafficking. GCaMP6M is an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with medium decay and response kinetics. Of note, the allele was designed with an IRES upstream of the tTA2 to reduce/eliminate leakiness in the absence of Cre recombinase.
(J:101977)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Igs7 Mutation: |
66 strains or lines available
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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