Igs7^{tm230(tetO-XCaMPG,CAG-ChRmine*/oScarlet*,-tTA2)Daigl}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm230(tetO-XCaMPG,CAG-ChRmine*/oScarlet*,-tTA2)Daigl}
• Name: intergenic site 7; targeted mutation 230, Tanya Daigle
• MGI ID: MGI:7482009
• Synonyms: Ai230, Ai230^{TIT2L-XCaMPG-WPRE-ICL-ChRmine-TS-oScarlet-Kv2.1-ER-IRES2-tTA2-WPRE_hyg}, Igs7^{tm230(tetO-XCaMPG,CAG-ChRmine*/oScarlet*,-tTA2)Tasic}
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm230(tetO-XCaMPG,CAG-ChRmine*/oScarlet*,-tTA2)Daigl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette, XCaMP-G-WPRE, a CAG promoter, Lox2272-STOP-Lox2272 cassette, ChRmine*/oScarlet* sequence, and IRES-tTA2-WPRE sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high cre and/or Tet inducibility). ChRmine is a pump-like cation-conducting channelrhodopsin, derived from Rhodomonas lens, that exhibits extremely high current and light sensitivity, as well as a markedly red-shifted spectrum. This is fused with a membrane trafficking signal (TS) and oScarlet fluorescent protein, which is based on the sequence of mScarlet with an additional E95D mutation to increase neuronal cell trafficking. XCaMP-G is an EGFP fluorescent calcium detector of neuronal activity with high signal-to-noise ratio, linear property, and high-frequency spike resolution suitable for in vivo interrogation of complex neural dynamics. XCaMP-G incorporates a second calmodulin-binding peptide as a "protection motif", which decreases calcium channel disruption and nuclear accumulation. Of note, the Ai229 allele was designed with an IRES upstream of the tTA2 sequence to reduce/eliminate leakiness in the absence of cre recombinase. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 70 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)