Vil1^{em1(flpo)Lbrl}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Vil1^{em1(flpo)Lbrl}
• Name: villin 1; endonuclease-mediated mutation 1, Stephen Liberles
• MGI ID: MGI:7482596
• Synonyms: Vil1-p2a-FlpO
• Gene: Vil1 Location: Chr1:74448543-74474719 bp, + strand Genetic Position: Chr1, 38.54 cM, cytoband C5
• Alliance: Vil1^{em1(flpo)Lbrl} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Vil1^{em1(flpo)Lbrl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Vil1 (MGI:98930)

• Mutation details: CRISPR/Cas9 methodologies were used to replace the Stop codon of the mouse Vil1 gene with p2A-FlpO recombinase sequence. (J:335608)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vil1 (mouse) Summary of all recombinase alleles driven by Vil1. Comparative matrix view of recombinase activities Alliance mouse Vil1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vil1 Mutation: 52 strains or lines available

References

• Original: J:335608 Hayashi M, et al., Enteroendocrine cell lineages that differentially control feeding and gut motility. Elife. 2023 Feb 22;12
• All: 1 reference(s)