Hlf^{em2(cre/ERT2)Suda}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hlf^{em2(cre/ERT2)Suda}
• Name: hepatic leukemia factor; endonuclease-mediated mutation 2, Toshio Suda
• MGI ID: MGI:7486479
• Synonyms: Hlf-Cre^ERT2
• Gene: Hlf Location: Chr11:90227362-90281721 bp, - strand Genetic Position: Chr11, 55.03 cM
• Alliance: Hlf^{em2(cre/ERT2)Suda} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hlf^{em2(cre/ERT2)Suda} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hlf (MGI:96108)

• Mutation details: Using the CRISPR/Cas9 method, a T2A-CreERT2 fusion gene was inserted before the endogenous stop codon within exon 4. (J:328986, J:336140)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hlf (mouse) Summary of all recombinase alleles driven by Hlf. Comparative matrix view of recombinase activities Alliance mouse Hlf gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hlf Mutation: 17 strains or lines available

References

• Original: J:336140 Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
• All: 2 reference(s)