Misp^em1Nsas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Misp^em1Nsas
• Name: mitotic spindle positioning; endonuclease-mediated mutation 1, Nobuya Sasaki
• MGI ID: MGI:7486552
• Synonyms: Misp KO
• Gene: Misp Location: Chr10:79656853-79666286 bp, + strand Genetic Position: Chr10, 39.72 cM, cytoband C1
• Alliance: Misp^em1Nsas page

Mutation origin

• Strain of Origin: FVB/NJcl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 5-base deletion (TGCAC) in exon 2 of the gene. This deletion leads to a frame-shift and a premature stop codon (p.S220Qfs*2). Western blot analysis confirmed the absence of protein expression in the colon of homozygous mutant mice. (J:333842)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Misp Mutation: 20 strains or lines available

References

• Original: J:333842 Hiura K, et al., Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice. J Vet Med Sci. 2023 Feb 1;85(2):167-174
• All: 1 reference(s)