Sema6a^{tm1(cre/ERT2)Alk}
Targeted Allele Detail

Summary

• Symbol: Sema6a^{tm1(cre/ERT2)Alk}
• Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; targeted mutation 1, Alex L Kolodkin
• MGI ID: MGI:7488666
• Synonyms: Sema6A^CreERT2
• Gene: Sema6a Location: Chr18:47378321-47504267 bp, - strand Genetic Position: Chr18, 25.08 cM, cytoband C
• Alliance: Sema6a^{tm1(cre/ERT2)Alk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328276
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Sema6a^{tm1(cre/ERT2)Alk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sema6a (MGI:1203727)

• Mutation details: The allele was generated by introducing an insert containing a RAKR furin cleavage site followed by a 2xV5 tag, a P2A linker, and the CreERT2 coding sequence and frt-flanked neo cassette into the Sema6A locus just downstream of exon 19 using homologous recombination embryonic stem cell technology. (J:328276, J:350002)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sema6a (mouse) Summary of all recombinase alleles driven by Sema6a. Comparative matrix view of recombinase activities Alliance mouse Sema6a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema6a Mutation: 85 strains or lines available

References

• Original: J:328276 Hunyara JL, et al., Characterization of non-alpha retinal ganglion cell injury responses reveals a possible block to restoring ipRGC function. Exp Neurol. 2022 Jul 20;357:114176
• All: 2 reference(s)