Rr50^em1Axvi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr50^em1Axvi
• Name: regulatory region 50; endonuclease-mediated mutation 1, Axel Visel
• MGI ID: MGI:7489592
• Synonyms: B6
• Gene: Rr50 Location: Chr12:35231324-35244624 bp Genetic Position: Chr12, Syntenic
• Alliance: Rr50^em1Axvi page

Mutation origin

• Strain of Origin: FVB

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intergenic deletion
• Mutation details: The topologically associating domain (TAD) boundary region or insulator between Snx13 and Ahr, separating the Twist1 TAD from the Ahr TAD, was targeted with sgRNAs (targeting GGGCACTGGGTTTCTTCTATCGG, TATTGCCTCAATGGAATAGGTGG, AGTAAGCAATGGTTTTGCATTGG and GTCTCTGACACTTGTTTGCTTGG) using CRISPR/Cas9 technology, resulting in a 13112 bp deletion (chr12:35231340-35244451(GRCm39)). (J:335096)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rr50 Mutation: 1 strain or line available

References

• Original: J:335096 Rajderkar S, et al., Topologically associating domain boundaries are required for normal genome function. Commun Biol. 2023 Apr 20;6(1):435
• All: 1 reference(s)