Tg(Bsx-icre)1Kess
Transgene Detail

Summary

• Symbol: Tg(Bsx-icre)1Kess
• Name: transgene insertion 1, Nicoletta Kessaris
• MGI ID: MGI:7490135
• Synonyms: Bsx-Cre
• Transgene: Tg(Bsx-icre)1Kess Location: unknown
• Alliance: Tg(Bsx-icre)1Kess page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Bsx-icre)1Kess expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Bsx (MGI:2669849)

• Mutation details: Homologous recombination in bacteria was used to modify a 180 Kb BAC RP24-255N16 containing 70 Kb upstream and 110 Kb downstream of the Bsx gene. The codon-improved Cre recombinase (iCre) conntaining a nuclear localization signal was fused to the initiation codon of the genes using a PCR-based approach. This was followed by a Simian Virus 40 polyadenylation signal and a Kanamycin resistance cassette that was flanked by FRT sites for the selection of recombinant BACs. BAC recombination was designed to delete the remaining ATG-encoding exon together with 50-200 bp from the downstream intron. Mice were generated by pronuclear injection of the construct into fertilized oocytes. No line number is given. (J:330767)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Bsx (mouse) Summary of all recombinase alleles driven by Bsx. Comparative matrix view of recombinase activities Alliance mouse Bsx gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:330767 Magno L, et al., Fate mapping reveals mixed embryonic origin and unique developmental codes of mouse forebrain septal neurons. Commun Biol. 2022 Oct 27;5(1):1137
• All: 1 reference(s)