Esm1tm1.1Rha
Targeted Allele Detail
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Symbol: |
Esm1tm1.1Rha |
Name: |
endothelial cell-specific molecule 1; targeted mutation 1.1, Ralf H Adams |
MGI ID: |
MGI:7490359 |
Synonyms: |
Esm1KO |
Gene: |
Esm1 Location: Chr13:113346193-113354632 bp, + strand Genetic Position: Chr13, 63.93 cM
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Alliance: |
Esm1tm1.1Rha page
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: LoxP recognition sites were inserted around the second exon of the murine Esm1 gene via homologous recombination in the ES cells. A murine cDNA fragment containing the coding sequence from exons 2 and 3 followed by 2 copies of the bovine growth hormone polyadenylation signal sequence was inserted in frame, and downstream of the loxP-flanked area, the targeting construct also contained a beta-galactosidase (lacZ) reporter cassette, which carried an artificial splice acceptor and an internal ribosomal entry site (IRES) at the 5-end and the SV40 polyadenylation signal sequence at the 3-end. The targeting construct also contained a neomycin resistance cassette surrounded by Frt sites allowing Flp recombinase-mediated removal. Through interbreeding with PGK-Cre deleter mice, exon 2 of the Esm1 gene was globally deleted. No beta-galactosidase reporter activity was detectable in heterozygous or homozygous mutants, and it was subsequently confirmed that Esm1-lacZ fusion transcripts were not detectable by qPCR.
(J:246778)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Esm1 Mutation: |
16 strains or lines available
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Original: |
J:246778 Rocha SF, et al., Esm1 modulates endothelial tip cell behavior and vascular permeability by enhancing VEGF bioavailability. Circ Res. 2014 Aug 29;115(6):581-90 |
All: |
1 reference(s) |
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