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Esm1tm1.1Rha
Targeted Allele Detail
Summary
Symbol: Esm1tm1.1Rha
Name: endothelial cell-specific molecule 1; targeted mutation 1.1, Ralf H Adams
MGI ID: MGI:7490359
Synonyms: Esm1KO
Gene: Esm1  Location: Chr13:113346193-113354632 bp, + strand  Genetic Position: Chr13, 63.93 cM
Alliance: Esm1tm1.1Rha page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:246778
Parent Cell Line:  F1H4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NTac)F1
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsLoxP recognition sites were inserted around the second exon of the murine Esm1 gene via homologous recombination in the ES cells. A murine cDNA fragment containing the coding sequence from exons 2 and 3 followed by 2 copies of the bovine growth hormone polyadenylation signal sequence was inserted in frame, and downstream of the loxP-flanked area, the targeting construct also contained a beta-galactosidase (lacZ) reporter cassette, which carried an artificial splice acceptor and an internal ribosomal entry site (IRES) at the 5-end and the SV40 polyadenylation signal sequence at the 3-end. The targeting construct also contained a neomycin resistance cassette surrounded by Frt sites allowing Flp recombinase-mediated removal. Through interbreeding with PGK-Cre deleter mice, exon 2 of the Esm1 gene was globally deleted. No beta-galactosidase reporter activity was detectable in heterozygous or homozygous mutants, and it was subsequently confirmed that Esm1-lacZ fusion transcripts were not detectable by qPCR. (J:246778)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Esm1 Mutation:  16 strains or lines available
References
Original:  J:246778 Rocha SF, et al., Esm1 modulates endothelial tip cell behavior and vascular permeability by enhancing VEGF bioavailability. Circ Res. 2014 Aug 29;115(6):581-90
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory