Rhoq^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rhoq^{tm1b(EUCOMM)Hmgu}
• Name: ras homolog family member Q; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7492305
• Synonyms: TC10^tm1b
• Gene: Rhoq Location: Chr17:87270539-87307497 bp, + strand Genetic Position: Chr17, 56.9 cM
• Alliance: Rhoq^{tm1b(EUCOMM)Hmgu} page
• IMPC: Rhoq gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308705
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 87271472 of Chromosome 17 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 87271906. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Immunoblot analysis confirmed the absence of protein expression in brain, liver, and lung homogenates isolated from adult homozygous mutant mice. (J:308705)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhoq Mutation: 16 strains or lines available

References

• Original: J:308705 Koinuma S, et al., TC10, a Rho family GTPase, is required for efficient axon regeneration in a neuron-autonomous manner. J Neurochem. 2021 May;157(4):1196-1206
• All: 1 reference(s)