Tmem249^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmem249^em1Osb
• Name: transmembrane protein 249; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:7495499
• Synonyms: Catsperq^-
• Gene: Tmem249 Location: Chr15:76417411-76419197 bp, - strand Genetic Position: Chr15, 36.03 cM
• Alliance: Tmem249^em1Osb page

Mutation origin

• Strain of Origin: B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate an allele with two simultaneous deletions: a 1,291bp deletion removing the region spanning the end of exon 1 to intron 5, and an 11bp deletion in exon 6. The deletions were expected to cause out-of-frame mutations, resulting in early termination of protein translation from the CTG start codon. Western blot and immunocytochemistry analyses confirmed the absence of encoded protein in homozygous null sperm. (J:337426)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem249 Mutation: 1 strain or line available

References

• Original: J:337426 Huang X, et al., A CUG-initiated CATSPERtheta functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking. bioRxiv. 2023 Mar 17
• All: 1 reference(s)