Tssk3^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tssk3^em1Osb
• Name: testis-specific serine kinase 3; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:7495884
• Synonyms: Tssk3 KO
• Gene: Tssk3 Location: Chr4:129382807-129384815 bp, - strand Genetic Position: Chr4, 63.26 cM, cytoband D2.3
• Alliance: Tssk3^em1Osb page

Mutation origin

• Strain of Origin: B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 1551 bp deletion in the gene, corresponding to the entire region encoding the kinase domain, and almost the entire protein coding region except the first 6 amino acids. Western blot analysis confirmed the absence of encoded protein in homozygous mutant testes. (J:337565)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tssk3 Mutation: 10 strains or lines available

References

• Original: J:337565 Nozawa K, et al., Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility. Andrology. 2023 Jul;11(5):826-839
• All: 1 reference(s)