Frey1^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Frey1^em1Osb
• Name: Frey regulator of sperm-oocyte fusion 1; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:7495885
• Synonyms: 1700029I15Rik^-, 1700029I15Rik^em1Osb
• Gene: Frey1 Location: Chr2:92213263-92213948 bp, + strand Genetic Position: Chr2, 50.99 cM
• Alliance: Frey1^em1Osb page

Mutation origin

• Strain of Origin: B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 619-bp deletion in the coding region, resulting in translation of an incorrect amino acid sequence. Western blot analysis confirmed the absence of protein expression in homozygous null testes. (J:337566)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Frey1 Mutation: 8 strains or lines available

References

• Original: J:337566 Lu Y, et al., 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2207263120
• All: 1 reference(s)