Flrt2em1Jnk
Endonuclease-mediated Allele Detail
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Symbol: |
Flrt2em1Jnk |
Name: |
fibronectin leucine rich transmembrane protein 2; endonuclease-mediated mutation 1, Jeremy Kay |
MGI ID: |
MGI:7505615 |
Synonyms: |
FLRT2H170N, FLRT2UF |
Gene: |
Flrt2 Location: Chr12:95659000-95751989 bp, + strand Genetic Position: Chr12, 46.44 cM
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Alliance: |
Flrt2em1Jnk page
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Allele Type: |
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Endonuclease-mediated (Not Applicable) |
Mutation: |
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Nucleotide substitutions
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Mutation details: CRISPR/cas9 methodologies are used to introduce an H170N (nucleotide change CAC to AAT, note AAC was originally reported) in exon 2. The mutation disrupts interactions between FLRT2 and its ligands UNC5C and UNC5D. A single guide RNA (sgRNA) was produced in which the PAM sequence overlaps the H170 codon (sequence 5' GCCCAACAGGCACGCTACTCagg; PAM site is denoted by lowercase letters). The PAM sequence is destroyed by the codon-altering mutation, thereby rendering correctly edited alleles immune to further cas9 cutting activity.
(J:339867)
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Original: |
J:339867 Prigge CL, et al., Rejection of inappropriate synaptic partners in mouse retina mediated by transcellular FLRT2-UNC5 signaling. Dev Cell. 2023 Aug 3; |
All: |
1 reference(s) |
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