Tsks^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tsks^em1Osb
• Name: testis-specific serine kinase substrate; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:7509242
• Synonyms: Tsks KO
• Gene: Tsks Location: Chr7:44592628-44607459 bp, + strand Genetic Position: Chr7, 29.07 cM
• Alliance: Tsks^em1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337844
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 14,832 bp deletion including exons 1-11 of the gene. Western blot analysis confirmed the absence of protein expression in the testes of homozygous mutant males. (J:337844)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tsks Mutation: 23 strains or lines available

References

• Original: J:337844 Shimada K, et al., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2221762120
• All: 1 reference(s)